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    Addgene inc tev addgene 8827 software
    Tev Addgene 8827 Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tev+addgene+8827+software/pRK793+(Plasmid+%238827)/pm39637853-249-157-158
    Average 95 stars, based on 212 article reviews
    tev addgene 8827 software - by Bioz Stars, 2026-09
    95/100 stars

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    Article Title: G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Thermo Scientific EC0114 Rosetta (DE3) pLysS Millipore Sigma 70956-3 Chemicals, peptides, and recombinant proteins Sodium (meta)arsenite Sigma-Aldrich S7400-100G Chloramphenicol Sigma-Aldrich C0378-25G Ampicillin Sigma-Aldrich A9518-25G Isopropyl-b-D-thiogalactopyranoside (IPTG) Fisher Scientific BP1755100 HEPES Fisher Scientific BP310-500 cOmplete Protease Inhibitor Cocktail, EDTA-Free, Mini, Tablets Sigma-Aldrich 11836170001 L-Glutathione reduced Sigma-Aldrich G4251-10G Ni-NTA Agarose Fisher Scientific R90115 Imidazole Fisher Scientific O3196-500 Triton X-100 Fisher Scientific AC327371000 Urea Sigma-Aldrich U1250-1KG DyLight 550 NHS Ester Thermo Scientific 62263 AfeI New England Biolabs R0652L BamHI-HF New England Biolabs R3136T Cyanine 5-UTP (enhanced) Enzo Life Sciences ENZ-42506 Cyanine 3-UTP (enhanced) Enzo Life Sciences ENZ-42505 Fluorescein 12 UTP Enzo Life Sciences ENZ-42834 TURBO DNase Invitrogen AM2239 Polyadenylic acid Roche 10108626001 Polycytidylic acid Sigma-Aldrich P4903 Polyuridylic acid Sigma-Aldrich P9528 Polyguanylic acid Sigma-Aldrich P4404 TRIzol Thermo Scientific 15596018 Polyethylene Glycol 3350 Fisher Scientific 18-605-319 PCR grade Proteinase K Thermo Scientific EO0491 Proteinase K, Molecular Biology Grade New England Biolabs P8107S RNase A Thermo Scientific EN0531 NuPAGE MES SDS Running Buffer (20X) Invitrogen NP0002 SimplyBlue SafeStain Invitrogen LC6065 Ethylenediaminetetraacetic acid Sigma-Aldrich E5134 Formamide Sigma-Aldrich 4650 1 6-Hexanediol Sigma-Aldrich 240117 4’-Aminomethyltrioxsalen hydrochloride Sigma-Aldrich A4330 Tricine Sigma-Aldrich T0377 Triethanolamine Sigma-Aldrich 90279 ssRNA ladder New England Biolabs N0362S Lipofectamine 3000 Thermo Scientific L3000008 Carbonyl cyanide 3-chlorophenylhydrazone Sigma-Aldrich C2759 2-Deoxy-D-glucose Santa Cruz Biotechnology sc-202010A G3Ia Gift from Taylor Lab Freibaum et al.53 (Continued on next page) Molecular Cell 85, 1–14.e1–e7, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER G3Ia’ Gift from Taylor Lab Freibaum et al.53 Tween 20 Sigma-Aldrich P9416 RNase I Thermo Scientific AM2294 Dextran sulfate Fisher Scientific S4030 E. coli tRNA Sigma-Aldrich R1753 Vanadyl Ribonucleoside Complex Sigma-Aldrich R3380 VECTAshield Vector Laboratories H-1000 Critical commercial assays Megascript Thermo Scientific AM1334 Megascript cleanup Thermo Scientific AM1908 Label IT Nucleic Acid Labeling Kit Mirus Bio MIR 3200; MIR 3600; MIR 3725 Whole Plasmid Sequencing Plasmidsaurus N/A Experimental models: Cell lines U-2 OS wt ATCC HTB-96 U-2-OS G3BP1-GFP Taylor lab Figley et al.70 U-2 OS mRuby-2-PABPC1 Parker lab Burke et al.58 Oligonucleotides See Table S1 for list of smFISH and smiFISH probe oligos Recombinant DNA GST-G3BP1 Yang et al.20 N/A GST-EGFP-G3BP1 Yang et al.20 N/A 6xHis-MEG-3 Smith et al.71 pDC20 T7 luciferase Promega L4741 T7 cycb Drosophila Genomics Resource Center 1295561 T7 ccr4 Drosophila Genomics Resource Center 4406 T7 pgc Drosophila Genomics Resource Center 8512 EGFP-NPM1 Addgene 1757861 EGFP-b-Actin Addgene 5642157 pRK793 7xHis-TEV Addgene 8827 Software and algorithms ImageJ/FIJI NIH https://imagej.nih.gov/ij/ MatLab Mathworks https://www.mathworks.com/ products/matlab.html FISHquant N/A https://fish-quant.github.io/ R CRAN https://cran.r-project.org/ R studio Posit https://posit.co/products/open- source/rstudio/ Softworx GE https://download.cytivalifesciences. com/cellanalysis/download_data/ softWoRx/7.2.1/ReleaseNotes_ softWoRx_721.html Deposited data Analysis scripts This paper Zenodo: https://doi.org/10.5281/zenodo.14027059 Other Vivaspin 20 MWCO 30 000 Cytiva 28-9323-61 GSTrap FF Cytiva 17-5131-02 (Continued on next page) e2 Molecular Cell 85, 1–14.e1–e7, February 6, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HiLoad 16/600 Superdex 200 pg Cytiva 28989335 Cytiva Whatman Uniflo Syringe Filters, 0.2 mm PES Fisher Scientific 09-928-062 Nupage 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well Thermo Scientific NP0322BOX Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL Fisher Scientific 89882 12 mM # 1.5 round glass cover slips Warner Instruments cs-12r15 24 x 30 mM # 1.5 glass cover slips Thomas Scientific CLS-1764-2430 Press-to-seal Silicone isolator Grace Biolabs GBL664504-25EA

    Plasmid Preparation:

    Article Title: G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Thermo Scientific EC0114 Rosetta (DE3) pLysS Millipore Sigma 70956-3 Chemicals, peptides, and recombinant proteins Sodium (meta)arsenite Sigma-Aldrich S7400-100G Chloramphenicol Sigma-Aldrich C0378-25G Ampicillin Sigma-Aldrich A9518-25G Isopropyl-b-D-thiogalactopyranoside (IPTG) Fisher Scientific BP1755100 HEPES Fisher Scientific BP310-500 cOmplete Protease Inhibitor Cocktail, EDTA-Free, Mini, Tablets Sigma-Aldrich 11836170001 L-Glutathione reduced Sigma-Aldrich G4251-10G Ni-NTA Agarose Fisher Scientific R90115 Imidazole Fisher Scientific O3196-500 Triton X-100 Fisher Scientific AC327371000 Urea Sigma-Aldrich U1250-1KG DyLight 550 NHS Ester Thermo Scientific 62263 AfeI New England Biolabs R0652L BamHI-HF New England Biolabs R3136T Cyanine 5-UTP (enhanced) Enzo Life Sciences ENZ-42506 Cyanine 3-UTP (enhanced) Enzo Life Sciences ENZ-42505 Fluorescein 12 UTP Enzo Life Sciences ENZ-42834 TURBO DNase Invitrogen AM2239 Polyadenylic acid Roche 10108626001 Polycytidylic acid Sigma-Aldrich P4903 Polyuridylic acid Sigma-Aldrich P9528 Polyguanylic acid Sigma-Aldrich P4404 TRIzol Thermo Scientific 15596018 Polyethylene Glycol 3350 Fisher Scientific 18-605-319 PCR grade Proteinase K Thermo Scientific EO0491 Proteinase K, Molecular Biology Grade New England Biolabs P8107S RNase A Thermo Scientific EN0531 NuPAGE MES SDS Running Buffer (20X) Invitrogen NP0002 SimplyBlue SafeStain Invitrogen LC6065 Ethylenediaminetetraacetic acid Sigma-Aldrich E5134 Formamide Sigma-Aldrich 4650 1 6-Hexanediol Sigma-Aldrich 240117 4’-Aminomethyltrioxsalen hydrochloride Sigma-Aldrich A4330 Tricine Sigma-Aldrich T0377 Triethanolamine Sigma-Aldrich 90279 ssRNA ladder New England Biolabs N0362S Lipofectamine 3000 Thermo Scientific L3000008 Carbonyl cyanide 3-chlorophenylhydrazone Sigma-Aldrich C2759 2-Deoxy-D-glucose Santa Cruz Biotechnology sc-202010A G3Ia Gift from Taylor Lab Freibaum et al.53 (Continued on next page) Molecular Cell 85, 1–14.e1–e7, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER G3Ia’ Gift from Taylor Lab Freibaum et al.53 Tween 20 Sigma-Aldrich P9416 RNase I Thermo Scientific AM2294 Dextran sulfate Fisher Scientific S4030 E. coli tRNA Sigma-Aldrich R1753 Vanadyl Ribonucleoside Complex Sigma-Aldrich R3380 VECTAshield Vector Laboratories H-1000 Critical commercial assays Megascript Thermo Scientific AM1334 Megascript cleanup Thermo Scientific AM1908 Label IT Nucleic Acid Labeling Kit Mirus Bio MIR 3200; MIR 3600; MIR 3725 Whole Plasmid Sequencing Plasmidsaurus N/A Experimental models: Cell lines U-2 OS wt ATCC HTB-96 U-2-OS G3BP1-GFP Taylor lab Figley et al.70 U-2 OS mRuby-2-PABPC1 Parker lab Burke et al.58 Oligonucleotides See Table S1 for list of smFISH and smiFISH probe oligos Recombinant DNA GST-G3BP1 Yang et al.20 N/A GST-EGFP-G3BP1 Yang et al.20 N/A 6xHis-MEG-3 Smith et al.71 pDC20 T7 luciferase Promega L4741 T7 cycb Drosophila Genomics Resource Center 1295561 T7 ccr4 Drosophila Genomics Resource Center 4406 T7 pgc Drosophila Genomics Resource Center 8512 EGFP-NPM1 Addgene 1757861 EGFP-b-Actin Addgene 5642157 pRK793 7xHis-TEV Addgene 8827 Software and algorithms ImageJ/FIJI NIH https://imagej.nih.gov/ij/ MatLab Mathworks https://www.mathworks.com/ products/matlab.html FISHquant N/A https://fish-quant.github.io/ R CRAN https://cran.r-project.org/ R studio Posit https://posit.co/products/open- source/rstudio/ Softworx GE https://download.cytivalifesciences. com/cellanalysis/download_data/ softWoRx/7.2.1/ReleaseNotes_ softWoRx_721.html Deposited data Analysis scripts This paper Zenodo: https://doi.org/10.5281/zenodo.14027059 Other Vivaspin 20 MWCO 30 000 Cytiva 28-9323-61 GSTrap FF Cytiva 17-5131-02 (Continued on next page) e2 Molecular Cell 85, 1–14.e1–e7, February 6, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HiLoad 16/600 Superdex 200 pg Cytiva 28989335 Cytiva Whatman Uniflo Syringe Filters, 0.2 mm PES Fisher Scientific 09-928-062 Nupage 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well Thermo Scientific NP0322BOX Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL Fisher Scientific 89882 12 mM # 1.5 round glass cover slips Warner Instruments cs-12r15 24 x 30 mM # 1.5 glass cover slips Thomas Scientific CLS-1764-2430 Press-to-seal Silicone isolator Grace Biolabs GBL664504-25EA

    Sequencing:

    Article Title: G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Thermo Scientific EC0114 Rosetta (DE3) pLysS Millipore Sigma 70956-3 Chemicals, peptides, and recombinant proteins Sodium (meta)arsenite Sigma-Aldrich S7400-100G Chloramphenicol Sigma-Aldrich C0378-25G Ampicillin Sigma-Aldrich A9518-25G Isopropyl-b-D-thiogalactopyranoside (IPTG) Fisher Scientific BP1755100 HEPES Fisher Scientific BP310-500 cOmplete Protease Inhibitor Cocktail, EDTA-Free, Mini, Tablets Sigma-Aldrich 11836170001 L-Glutathione reduced Sigma-Aldrich G4251-10G Ni-NTA Agarose Fisher Scientific R90115 Imidazole Fisher Scientific O3196-500 Triton X-100 Fisher Scientific AC327371000 Urea Sigma-Aldrich U1250-1KG DyLight 550 NHS Ester Thermo Scientific 62263 AfeI New England Biolabs R0652L BamHI-HF New England Biolabs R3136T Cyanine 5-UTP (enhanced) Enzo Life Sciences ENZ-42506 Cyanine 3-UTP (enhanced) Enzo Life Sciences ENZ-42505 Fluorescein 12 UTP Enzo Life Sciences ENZ-42834 TURBO DNase Invitrogen AM2239 Polyadenylic acid Roche 10108626001 Polycytidylic acid Sigma-Aldrich P4903 Polyuridylic acid Sigma-Aldrich P9528 Polyguanylic acid Sigma-Aldrich P4404 TRIzol Thermo Scientific 15596018 Polyethylene Glycol 3350 Fisher Scientific 18-605-319 PCR grade Proteinase K Thermo Scientific EO0491 Proteinase K, Molecular Biology Grade New England Biolabs P8107S RNase A Thermo Scientific EN0531 NuPAGE MES SDS Running Buffer (20X) Invitrogen NP0002 SimplyBlue SafeStain Invitrogen LC6065 Ethylenediaminetetraacetic acid Sigma-Aldrich E5134 Formamide Sigma-Aldrich 4650 1 6-Hexanediol Sigma-Aldrich 240117 4’-Aminomethyltrioxsalen hydrochloride Sigma-Aldrich A4330 Tricine Sigma-Aldrich T0377 Triethanolamine Sigma-Aldrich 90279 ssRNA ladder New England Biolabs N0362S Lipofectamine 3000 Thermo Scientific L3000008 Carbonyl cyanide 3-chlorophenylhydrazone Sigma-Aldrich C2759 2-Deoxy-D-glucose Santa Cruz Biotechnology sc-202010A G3Ia Gift from Taylor Lab Freibaum et al.53 (Continued on next page) Molecular Cell 85, 1–14.e1–e7, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER G3Ia’ Gift from Taylor Lab Freibaum et al.53 Tween 20 Sigma-Aldrich P9416 RNase I Thermo Scientific AM2294 Dextran sulfate Fisher Scientific S4030 E. coli tRNA Sigma-Aldrich R1753 Vanadyl Ribonucleoside Complex Sigma-Aldrich R3380 VECTAshield Vector Laboratories H-1000 Critical commercial assays Megascript Thermo Scientific AM1334 Megascript cleanup Thermo Scientific AM1908 Label IT Nucleic Acid Labeling Kit Mirus Bio MIR 3200; MIR 3600; MIR 3725 Whole Plasmid Sequencing Plasmidsaurus N/A Experimental models: Cell lines U-2 OS wt ATCC HTB-96 U-2-OS G3BP1-GFP Taylor lab Figley et al.70 U-2 OS mRuby-2-PABPC1 Parker lab Burke et al.58 Oligonucleotides See Table S1 for list of smFISH and smiFISH probe oligos Recombinant DNA GST-G3BP1 Yang et al.20 N/A GST-EGFP-G3BP1 Yang et al.20 N/A 6xHis-MEG-3 Smith et al.71 pDC20 T7 luciferase Promega L4741 T7 cycb Drosophila Genomics Resource Center 1295561 T7 ccr4 Drosophila Genomics Resource Center 4406 T7 pgc Drosophila Genomics Resource Center 8512 EGFP-NPM1 Addgene 1757861 EGFP-b-Actin Addgene 5642157 pRK793 7xHis-TEV Addgene 8827 Software and algorithms ImageJ/FIJI NIH https://imagej.nih.gov/ij/ MatLab Mathworks https://www.mathworks.com/ products/matlab.html FISHquant N/A https://fish-quant.github.io/ R CRAN https://cran.r-project.org/ R studio Posit https://posit.co/products/open- source/rstudio/ Softworx GE https://download.cytivalifesciences. com/cellanalysis/download_data/ softWoRx/7.2.1/ReleaseNotes_ softWoRx_721.html Deposited data Analysis scripts This paper Zenodo: https://doi.org/10.5281/zenodo.14027059 Other Vivaspin 20 MWCO 30 000 Cytiva 28-9323-61 GSTrap FF Cytiva 17-5131-02 (Continued on next page) e2 Molecular Cell 85, 1–14.e1–e7, February 6, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HiLoad 16/600 Superdex 200 pg Cytiva 28989335 Cytiva Whatman Uniflo Syringe Filters, 0.2 mm PES Fisher Scientific 09-928-062 Nupage 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well Thermo Scientific NP0322BOX Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL Fisher Scientific 89882 12 mM # 1.5 round glass cover slips Warner Instruments cs-12r15 24 x 30 mM # 1.5 glass cover slips Thomas Scientific CLS-1764-2430 Press-to-seal Silicone isolator Grace Biolabs GBL664504-25EA

    Recombinant:

    Article Title: G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Thermo Scientific EC0114 Rosetta (DE3) pLysS Millipore Sigma 70956-3 Chemicals, peptides, and recombinant proteins Sodium (meta)arsenite Sigma-Aldrich S7400-100G Chloramphenicol Sigma-Aldrich C0378-25G Ampicillin Sigma-Aldrich A9518-25G Isopropyl-b-D-thiogalactopyranoside (IPTG) Fisher Scientific BP1755100 HEPES Fisher Scientific BP310-500 cOmplete Protease Inhibitor Cocktail, EDTA-Free, Mini, Tablets Sigma-Aldrich 11836170001 L-Glutathione reduced Sigma-Aldrich G4251-10G Ni-NTA Agarose Fisher Scientific R90115 Imidazole Fisher Scientific O3196-500 Triton X-100 Fisher Scientific AC327371000 Urea Sigma-Aldrich U1250-1KG DyLight 550 NHS Ester Thermo Scientific 62263 AfeI New England Biolabs R0652L BamHI-HF New England Biolabs R3136T Cyanine 5-UTP (enhanced) Enzo Life Sciences ENZ-42506 Cyanine 3-UTP (enhanced) Enzo Life Sciences ENZ-42505 Fluorescein 12 UTP Enzo Life Sciences ENZ-42834 TURBO DNase Invitrogen AM2239 Polyadenylic acid Roche 10108626001 Polycytidylic acid Sigma-Aldrich P4903 Polyuridylic acid Sigma-Aldrich P9528 Polyguanylic acid Sigma-Aldrich P4404 TRIzol Thermo Scientific 15596018 Polyethylene Glycol 3350 Fisher Scientific 18-605-319 PCR grade Proteinase K Thermo Scientific EO0491 Proteinase K, Molecular Biology Grade New England Biolabs P8107S RNase A Thermo Scientific EN0531 NuPAGE MES SDS Running Buffer (20X) Invitrogen NP0002 SimplyBlue SafeStain Invitrogen LC6065 Ethylenediaminetetraacetic acid Sigma-Aldrich E5134 Formamide Sigma-Aldrich 4650 1 6-Hexanediol Sigma-Aldrich 240117 4’-Aminomethyltrioxsalen hydrochloride Sigma-Aldrich A4330 Tricine Sigma-Aldrich T0377 Triethanolamine Sigma-Aldrich 90279 ssRNA ladder New England Biolabs N0362S Lipofectamine 3000 Thermo Scientific L3000008 Carbonyl cyanide 3-chlorophenylhydrazone Sigma-Aldrich C2759 2-Deoxy-D-glucose Santa Cruz Biotechnology sc-202010A G3Ia Gift from Taylor Lab Freibaum et al.53 (Continued on next page) Molecular Cell 85, 1–14.e1–e7, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER G3Ia’ Gift from Taylor Lab Freibaum et al.53 Tween 20 Sigma-Aldrich P9416 RNase I Thermo Scientific AM2294 Dextran sulfate Fisher Scientific S4030 E. coli tRNA Sigma-Aldrich R1753 Vanadyl Ribonucleoside Complex Sigma-Aldrich R3380 VECTAshield Vector Laboratories H-1000 Critical commercial assays Megascript Thermo Scientific AM1334 Megascript cleanup Thermo Scientific AM1908 Label IT Nucleic Acid Labeling Kit Mirus Bio MIR 3200; MIR 3600; MIR 3725 Whole Plasmid Sequencing Plasmidsaurus N/A Experimental models: Cell lines U-2 OS wt ATCC HTB-96 U-2-OS G3BP1-GFP Taylor lab Figley et al.70 U-2 OS mRuby-2-PABPC1 Parker lab Burke et al.58 Oligonucleotides See Table S1 for list of smFISH and smiFISH probe oligos Recombinant DNA GST-G3BP1 Yang et al.20 N/A GST-EGFP-G3BP1 Yang et al.20 N/A 6xHis-MEG-3 Smith et al.71 pDC20 T7 luciferase Promega L4741 T7 cycb Drosophila Genomics Resource Center 1295561 T7 ccr4 Drosophila Genomics Resource Center 4406 T7 pgc Drosophila Genomics Resource Center 8512 EGFP-NPM1 Addgene 1757861 EGFP-b-Actin Addgene 5642157 pRK793 7xHis-TEV Addgene 8827 Software and algorithms ImageJ/FIJI NIH https://imagej.nih.gov/ij/ MatLab Mathworks https://www.mathworks.com/ products/matlab.html FISHquant N/A https://fish-quant.github.io/ R CRAN https://cran.r-project.org/ R studio Posit https://posit.co/products/open- source/rstudio/ Softworx GE https://download.cytivalifesciences. com/cellanalysis/download_data/ softWoRx/7.2.1/ReleaseNotes_ softWoRx_721.html Deposited data Analysis scripts This paper Zenodo: https://doi.org/10.5281/zenodo.14027059 Other Vivaspin 20 MWCO 30 000 Cytiva 28-9323-61 GSTrap FF Cytiva 17-5131-02 (Continued on next page) e2 Molecular Cell 85, 1–14.e1–e7, February 6, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HiLoad 16/600 Superdex 200 pg Cytiva 28989335 Cytiva Whatman Uniflo Syringe Filters, 0.2 mm PES Fisher Scientific 09-928-062 Nupage 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well Thermo Scientific NP0322BOX Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL Fisher Scientific 89882 12 mM # 1.5 round glass cover slips Warner Instruments cs-12r15 24 x 30 mM # 1.5 glass cover slips Thomas Scientific CLS-1764-2430 Press-to-seal Silicone isolator Grace Biolabs GBL664504-25EA

    Luciferase:

    Article Title: G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Thermo Scientific EC0114 Rosetta (DE3) pLysS Millipore Sigma 70956-3 Chemicals, peptides, and recombinant proteins Sodium (meta)arsenite Sigma-Aldrich S7400-100G Chloramphenicol Sigma-Aldrich C0378-25G Ampicillin Sigma-Aldrich A9518-25G Isopropyl-b-D-thiogalactopyranoside (IPTG) Fisher Scientific BP1755100 HEPES Fisher Scientific BP310-500 cOmplete Protease Inhibitor Cocktail, EDTA-Free, Mini, Tablets Sigma-Aldrich 11836170001 L-Glutathione reduced Sigma-Aldrich G4251-10G Ni-NTA Agarose Fisher Scientific R90115 Imidazole Fisher Scientific O3196-500 Triton X-100 Fisher Scientific AC327371000 Urea Sigma-Aldrich U1250-1KG DyLight 550 NHS Ester Thermo Scientific 62263 AfeI New England Biolabs R0652L BamHI-HF New England Biolabs R3136T Cyanine 5-UTP (enhanced) Enzo Life Sciences ENZ-42506 Cyanine 3-UTP (enhanced) Enzo Life Sciences ENZ-42505 Fluorescein 12 UTP Enzo Life Sciences ENZ-42834 TURBO DNase Invitrogen AM2239 Polyadenylic acid Roche 10108626001 Polycytidylic acid Sigma-Aldrich P4903 Polyuridylic acid Sigma-Aldrich P9528 Polyguanylic acid Sigma-Aldrich P4404 TRIzol Thermo Scientific 15596018 Polyethylene Glycol 3350 Fisher Scientific 18-605-319 PCR grade Proteinase K Thermo Scientific EO0491 Proteinase K, Molecular Biology Grade New England Biolabs P8107S RNase A Thermo Scientific EN0531 NuPAGE MES SDS Running Buffer (20X) Invitrogen NP0002 SimplyBlue SafeStain Invitrogen LC6065 Ethylenediaminetetraacetic acid Sigma-Aldrich E5134 Formamide Sigma-Aldrich 4650 1 6-Hexanediol Sigma-Aldrich 240117 4’-Aminomethyltrioxsalen hydrochloride Sigma-Aldrich A4330 Tricine Sigma-Aldrich T0377 Triethanolamine Sigma-Aldrich 90279 ssRNA ladder New England Biolabs N0362S Lipofectamine 3000 Thermo Scientific L3000008 Carbonyl cyanide 3-chlorophenylhydrazone Sigma-Aldrich C2759 2-Deoxy-D-glucose Santa Cruz Biotechnology sc-202010A G3Ia Gift from Taylor Lab Freibaum et al.53 (Continued on next page) Molecular Cell 85, 1–14.e1–e7, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER G3Ia’ Gift from Taylor Lab Freibaum et al.53 Tween 20 Sigma-Aldrich P9416 RNase I Thermo Scientific AM2294 Dextran sulfate Fisher Scientific S4030 E. coli tRNA Sigma-Aldrich R1753 Vanadyl Ribonucleoside Complex Sigma-Aldrich R3380 VECTAshield Vector Laboratories H-1000 Critical commercial assays Megascript Thermo Scientific AM1334 Megascript cleanup Thermo Scientific AM1908 Label IT Nucleic Acid Labeling Kit Mirus Bio MIR 3200; MIR 3600; MIR 3725 Whole Plasmid Sequencing Plasmidsaurus N/A Experimental models: Cell lines U-2 OS wt ATCC HTB-96 U-2-OS G3BP1-GFP Taylor lab Figley et al.70 U-2 OS mRuby-2-PABPC1 Parker lab Burke et al.58 Oligonucleotides See Table S1 for list of smFISH and smiFISH probe oligos Recombinant DNA GST-G3BP1 Yang et al.20 N/A GST-EGFP-G3BP1 Yang et al.20 N/A 6xHis-MEG-3 Smith et al.71 pDC20 T7 luciferase Promega L4741 T7 cycb Drosophila Genomics Resource Center 1295561 T7 ccr4 Drosophila Genomics Resource Center 4406 T7 pgc Drosophila Genomics Resource Center 8512 EGFP-NPM1 Addgene 1757861 EGFP-b-Actin Addgene 5642157 pRK793 7xHis-TEV Addgene 8827 Software and algorithms ImageJ/FIJI NIH https://imagej.nih.gov/ij/ MatLab Mathworks https://www.mathworks.com/ products/matlab.html FISHquant N/A https://fish-quant.github.io/ R CRAN https://cran.r-project.org/ R studio Posit https://posit.co/products/open- source/rstudio/ Softworx GE https://download.cytivalifesciences. com/cellanalysis/download_data/ softWoRx/7.2.1/ReleaseNotes_ softWoRx_721.html Deposited data Analysis scripts This paper Zenodo: https://doi.org/10.5281/zenodo.14027059 Other Vivaspin 20 MWCO 30 000 Cytiva 28-9323-61 GSTrap FF Cytiva 17-5131-02 (Continued on next page) e2 Molecular Cell 85, 1–14.e1–e7, February 6, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HiLoad 16/600 Superdex 200 pg Cytiva 28989335 Cytiva Whatman Uniflo Syringe Filters, 0.2 mm PES Fisher Scientific 09-928-062 Nupage 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well Thermo Scientific NP0322BOX Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL Fisher Scientific 89882 12 mM # 1.5 round glass cover slips Warner Instruments cs-12r15 24 x 30 mM # 1.5 glass cover slips Thomas Scientific CLS-1764-2430 Press-to-seal Silicone isolator Grace Biolabs GBL664504-25EA

    Software:

    Article Title: G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) Thermo Scientific EC0114 Rosetta (DE3) pLysS Millipore Sigma 70956-3 Chemicals, peptides, and recombinant proteins Sodium (meta)arsenite Sigma-Aldrich S7400-100G Chloramphenicol Sigma-Aldrich C0378-25G Ampicillin Sigma-Aldrich A9518-25G Isopropyl-b-D-thiogalactopyranoside (IPTG) Fisher Scientific BP1755100 HEPES Fisher Scientific BP310-500 cOmplete Protease Inhibitor Cocktail, EDTA-Free, Mini, Tablets Sigma-Aldrich 11836170001 L-Glutathione reduced Sigma-Aldrich G4251-10G Ni-NTA Agarose Fisher Scientific R90115 Imidazole Fisher Scientific O3196-500 Triton X-100 Fisher Scientific AC327371000 Urea Sigma-Aldrich U1250-1KG DyLight 550 NHS Ester Thermo Scientific 62263 AfeI New England Biolabs R0652L BamHI-HF New England Biolabs R3136T Cyanine 5-UTP (enhanced) Enzo Life Sciences ENZ-42506 Cyanine 3-UTP (enhanced) Enzo Life Sciences ENZ-42505 Fluorescein 12 UTP Enzo Life Sciences ENZ-42834 TURBO DNase Invitrogen AM2239 Polyadenylic acid Roche 10108626001 Polycytidylic acid Sigma-Aldrich P4903 Polyuridylic acid Sigma-Aldrich P9528 Polyguanylic acid Sigma-Aldrich P4404 TRIzol Thermo Scientific 15596018 Polyethylene Glycol 3350 Fisher Scientific 18-605-319 PCR grade Proteinase K Thermo Scientific EO0491 Proteinase K, Molecular Biology Grade New England Biolabs P8107S RNase A Thermo Scientific EN0531 NuPAGE MES SDS Running Buffer (20X) Invitrogen NP0002 SimplyBlue SafeStain Invitrogen LC6065 Ethylenediaminetetraacetic acid Sigma-Aldrich E5134 Formamide Sigma-Aldrich 4650 1 6-Hexanediol Sigma-Aldrich 240117 4’-Aminomethyltrioxsalen hydrochloride Sigma-Aldrich A4330 Tricine Sigma-Aldrich T0377 Triethanolamine Sigma-Aldrich 90279 ssRNA ladder New England Biolabs N0362S Lipofectamine 3000 Thermo Scientific L3000008 Carbonyl cyanide 3-chlorophenylhydrazone Sigma-Aldrich C2759 2-Deoxy-D-glucose Santa Cruz Biotechnology sc-202010A G3Ia Gift from Taylor Lab Freibaum et al.53 (Continued on next page) Molecular Cell 85, 1–14.e1–e7, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER G3Ia’ Gift from Taylor Lab Freibaum et al.53 Tween 20 Sigma-Aldrich P9416 RNase I Thermo Scientific AM2294 Dextran sulfate Fisher Scientific S4030 E. coli tRNA Sigma-Aldrich R1753 Vanadyl Ribonucleoside Complex Sigma-Aldrich R3380 VECTAshield Vector Laboratories H-1000 Critical commercial assays Megascript Thermo Scientific AM1334 Megascript cleanup Thermo Scientific AM1908 Label IT Nucleic Acid Labeling Kit Mirus Bio MIR 3200; MIR 3600; MIR 3725 Whole Plasmid Sequencing Plasmidsaurus N/A Experimental models: Cell lines U-2 OS wt ATCC HTB-96 U-2-OS G3BP1-GFP Taylor lab Figley et al.70 U-2 OS mRuby-2-PABPC1 Parker lab Burke et al.58 Oligonucleotides See Table S1 for list of smFISH and smiFISH probe oligos Recombinant DNA GST-G3BP1 Yang et al.20 N/A GST-EGFP-G3BP1 Yang et al.20 N/A 6xHis-MEG-3 Smith et al.71 pDC20 T7 luciferase Promega L4741 T7 cycb Drosophila Genomics Resource Center 1295561 T7 ccr4 Drosophila Genomics Resource Center 4406 T7 pgc Drosophila Genomics Resource Center 8512 EGFP-NPM1 Addgene 1757861 EGFP-b-Actin Addgene 5642157 pRK793 7xHis-TEV Addgene 8827 Software and algorithms ImageJ/FIJI NIH https://imagej.nih.gov/ij/ MatLab Mathworks https://www.mathworks.com/ products/matlab.html FISHquant N/A https://fish-quant.github.io/ R CRAN https://cran.r-project.org/ R studio Posit https://posit.co/products/open- source/rstudio/ Softworx GE https://download.cytivalifesciences. com/cellanalysis/download_data/ softWoRx/7.2.1/ReleaseNotes_ softWoRx_721.html Deposited data Analysis scripts This paper Zenodo: https://doi.org/10.5281/zenodo.14027059 Other Vivaspin 20 MWCO 30 000 Cytiva 28-9323-61 GSTrap FF Cytiva 17-5131-02 (Continued on next page) e2 Molecular Cell 85, 1–14.e1–e7, February 6, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HiLoad 16/600 Superdex 200 pg Cytiva 28989335 Cytiva Whatman Uniflo Syringe Filters, 0.2 mm PES Fisher Scientific 09-928-062 Nupage 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well Thermo Scientific NP0322BOX Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL Fisher Scientific 89882 12 mM # 1.5 round glass cover slips Warner Instruments cs-12r15 24 x 30 mM # 1.5 glass cover slips Thomas Scientific CLS-1764-2430 Press-to-seal Silicone isolator Grace Biolabs GBL664504-25EA



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